Human iPSC-derived Airway plates (cells in ALI formats)

Details

1. Robust recapitulation of human airway morphology as well as gene/protein expression and function (Inc. Cilia), for which functionally relevant assays can be used.
2. Reproduction of human airway pathologies well, including SARS-CoV-2 and flu infection and long-term inhalant/pollutant chemical exposure.
3. Strong epithelial barrier, which is highly advantageous for performing TEER assay.

Human iPSC-derived Airway plates (cells in multi-well)

Details

1. iPSC-derived airway epithelial cells are in ready-to-use multi-well format.
2. Highly suitable for higher throughput assays such as drug screening.
3. Great scalability with very limited well-to-well/lot-to-lot variability utilizing our proprietary iPSC-based technologies.
4. Reproduction of human airway pathologies well, including SARS-CoV-2 and flu infection.
5. Useful for concurrently evaluating airway toxicity of multiple toxicants.

Human iPSC-derived Alveolar plates (cells in ALI formats)

Details

1. Recapitulate human alveolar gene/protein expression, reproducing both type 1 and 2 cells orphologically accurately.
2. Reproduce human alveolar pathologies well, including SARS-CoV-2 infection and human lung toxicities.

REFERENCES

1. Yin X, Riva L, Pu Y, Martin-Sancho L, Kanamune J, Yamamoto Y, et al.
   「Cell Reports」 2020; 34: 108628.
   https://www.cell.com/cell-reports/pdf/S2211-1247(20)31617-X.pdf
2. Izumi Kimura, Daichi Yamasoba, Tomokazu Tamura, Naganori Nao, Tateki Suzuki, Yoshitaka Oda,… Kei Sato. (2022)
   「Cell」online on September 13,2022
   https://www.cell.com/cell/fulltext/S0092-8674(22)01190-4

MORE INFORMATION FOR Cell Products

1. Multi-well plates and ALI (air-liquid interface) of airway &alveolar cells available; organoids constituted from the same cells also available if right conditions are met.
2. Plates can be available on-demand or as subscription.
3. Standardized unaffected control cell plates readily available.
4. Patient-origin organoids available on demand -inquiry welcome.
5. Airway vs Alveolar comparison (e.g., SARS-CoV-2 variants) can be conducted reliably and reproducibly.
6. Overseas shipping available from our main lab in Kyoto, Japan.
7. Users in top academia and pharmaceuticals worldwide.
8. Contact us for customized/fit-for-purpose respiratory cell & organoid generation from patient-origin iPSCs.
9. Inquiries on pricing and other details very much welcome.
   contact@hilung.com

Our cells’ and organoids’ morphological manipulability, durability & scalability can be leveraged for high throughput screening.
Unaffected control cells/organoids for generic toxicity studies, as well as patient-derived and/or ene-edited cells/organoids for more specific mechanistic studies.
High-content analysis available as well, for detailed elucidation of human-relevant toxicity.

Our human iPSC-derived respiratory cells express essentially similar markers as their primary counterparts, allowing for reproducible and scalable human infection assays.
Validated markers include ACE and TMPRSS2, thus realizing a manipulable yet predictive tool with which SARS-CoV-2 infection can be efficiently and effectively assessed.
Other pathogens, including influenza and RSV, predictably infect our respiratory cells & organoids, after which we can utilize various assays.

Our alveolar organoids reproducibly demon-strate dynamic responses to pro-fibrotic and anti-fibrotic agents, leveraging the stable and unique form factor, and our 'swelling assay’.
Our antifibrotic re-expansion assay allows for non-destructive longitudinal tracking of dynamic, multi-faceted pathophysiology underlying pulmonary fibrosis.
Various conventional assays, as well as additional cellular/organoid manipulations, can be used in combination (e.g., patient-derived, genetic pulmonary fibrosis).

For further information, please contact us.

MORE INFORMATION FOR CRO Services

1. Airway & Alveolar models in full use.
2. Epithelium + fibroblast organoids available, with other multilineage organoids/MPS currently under development.
3. Open to patient-derived organoid generation.
4. Academic/non-profit discounts available; collaborative research on novel investigative efforts welcome.

Contact us for your challengeable research with our unique cells & organoids.
Inquiries on pricing and other details very much welcome.
contact@hilung.com
Thank you for your interest in our cell products and services!